Question:
I am interested in your N and C-terminal sequencing using MALDI ISD. How do I prepare the sample for analysis?
Answer:
MALDI ISD is used for confirmation of the N- and C-term of know proteins therefore you would need to supply us with the sequence of the protein you are working with so that we can confirm the sequence of the two termini. We require the samples to be in liquid form with a purity >80%. We need 20 ug of material to do the analysis if you can provide more this would be ideal. The more material we receive the better the quality of the results will be.
When you submit your samples you should list what is in the buffer you are using. Please let us know if there is glycerol in the sample as we need to remove this before placing the sample in the mass spec. The sample should be purified by reversed-phase HPLC.
You should note that the N- and C-terminal sequencing by MALDI ISD is done simultaneously on each sample.
Read more about N- and C-terminal sequencing using MALDI In Source Decay (ISD) in our application note, and about the Alphalyse N- and C-terminal sequencing service
Filed under: Uncategorized Tagged: | Alphalyse, blocked protein, C-terminal, c-terminal sequencing, in source decay, MALDI, maldi isd, mass spec, mass spec method, mass spectroscopy, N-terminal, N-terminal sequencing, Protein Analysis, Protein Mass Spec, protein service, proteomics, pyroglutamate
