PICK ‘n POST are fast and high-quality protein analysis services offered to research scientists in biotech, pharma and academia.
Your samples are easily prepared, packed and conveniently shipped by express courier using the PICK ‘n POST kit. A detailed result report is returned to you within 2 to 8 days depending on the service selected.
Choose from these easy to use services:
1. Protein Identification of bands & spots from 1D/2D SDS PAGE gels.
2. N-terminal Edman Sequencing of proteins and peptides.
3. Amino Acid Analysis of proteins, peptides & free Amino Acids.
4. Molecular Weight Determination of proteins and peptides.
All services are conducted at a fixed fee-per-service. We pick up your samples in your lab as part of the Pick ‘n Post service. The Pick ‘n Post™ kits are supplied free of charge and contain all the information and materials needed for easy and secure shipment of samples to Alphalyse.
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HIIIIII
i want to remove Tween 8o from a Human tPA protein. I know i can do it by dialysis . How can i actually do that and most imp which buffer i should use??????
Hi Mohammad
Tween 80 (http://en.wikipedia.org/wiki/Polysorbate_80) is nonionic and can be removed from charged protein and peptide samples by ion exchange chromatography. The choice of resin and buffer depends on protein amount and subsequent analysis. For mass spectrometric analysis at Alphalyse we typically use SCX material, for example SCX ZipTips from Millipore, with loading, washing, and elution buffers as described by Millipore.
BR
Ejvind, COO, Alphalyse