BLOG_PROTEIN_ANALYSIS_NC_PX

Techniques for N- and C-terminal sequencing of proteins

by Ejvind Mørtz, Ph.D in Protein Chemistry, COO at Alphalyse Where does the amino acid sequence start and where does it end? The terminal amino acid sequences are important for the biological functions of a protein and influence the protein

BLOG_PROTEIN_ANALYSIS_NC_PX

Techniques for N- and C-terminal sequencing of proteins

by Ejvind Mørtz, Ph.D in Protein Chemistry, COO at Alphalyse Where does the amino acid sequence start and where does it end? The terminal amino acid sequences are important for the biological functions of a protein and influence the protein

BLOG_PROTEIN_ANALYSIS_PX

How to increase protein concentration before loading onto 1D SDS PAGE?

Question: I have another question:  Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel? If not, how do you recommend  to reduce the volume of my sample?  Thanks Answer: There are different methods to increase the protein concentration

BLOG_PROTEIN_ANALYSIS_PX

How to increase protein concentration before loading onto 1D SDS PAGE?

Question: I have another question:  Is it ok if I precipitate my samples with TCA prior loading to the SDS-Gel? If not, how do you recommend  to reduce the volume of my sample?  Thanks Answer: There are different methods to increase the protein concentration

Identification of Proteolytic Degradation of recombinant protein?

Question: Hi there, I purified a recombinant protein and analyzed it by 1D SDS PAGE. In addition to the intact protein (52 kDa), there are 3 more bands presumably from proteolytical degradation of the protein. I would like to identify

Identification of Proteolytic Degradation of recombinant protein?

Question: Hi there, I purified a recombinant protein and analyzed it by 1D SDS PAGE. In addition to the intact protein (52 kDa), there are 3 more bands presumably from proteolytical degradation of the protein. I would like to identify

Mass spectrometric identification of proteins detected in Western Blots?

Question: Dear Sir, We have an unknown protein recognized in western blot by autoantibodies that we need to identify. Is it possible to send in a piece of a PVDF western bloting membrane for mass spectrometric protein identification, do you

Mass spectrometric identification of proteins detected in Western Blots?

Question: Dear Sir, We have an unknown protein recognized in western blot by autoantibodies that we need to identify. Is it possible to send in a piece of a PVDF western bloting membrane for mass spectrometric protein identification, do you

What buffer should I use for MALDI mass spectrometry analysis of my protein sample?

Although MALDI is more tolerant to contamination than other MS methods, the salts and detergents commonly used in biomolecular science can still interfere with either crystallisation or ionisation or both

What buffer should I use for MALDI mass spectrometry analysis of my protein sample?

Although MALDI is more tolerant to contamination than other MS methods, the salts and detergents commonly used in biomolecular science can still interfere with either crystallisation or ionisation or both

I just got my protein identification report. What does the Mascot Score mean?

The protein ID report contains the protein(s) that have been identified positively by their score in the Mascot database search. When we run MALDI mass spectroscopy and MS/MS the data is combined and searched in the database. The hits we get from the database are based on several proteins that are sequenced. Mascot shows which proteins could match the protein or proteins in each sample

I just got my protein identification report. What does the Mascot Score mean?

The protein ID report contains the protein(s) that have been identified positively by their score in the Mascot database search. When we run MALDI mass spectroscopy and MS/MS the data is combined and searched in the database. The hits we get from the database are based on several proteins that are sequenced. Mascot shows which proteins could match the protein or proteins in each sample

How do I prepare a sample for N- and C-terminal sequencing by MALDI In-Source-Decay (ISD)?

MALDI ISD is used for confirmation of the N- and C-term of know proteins therefore you would need to supply us with the sequence of the protein you are working with so that we can confirm the sequence of the two termini.

How do I prepare a sample for N- and C-terminal sequencing by MALDI In-Source-Decay (ISD)?

MALDI ISD is used for confirmation of the N- and C-term of know proteins therefore you would need to supply us with the sequence of the protein you are working with so that we can confirm the sequence of the two termini.